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Published online May 1, 2003
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McDonald, D. || Hope, T. J.

Submitted on March 7, 2003
Accepted on April 17, 2003

Recruitment of HIV and Its Receptors to Dendritic Cell-T Cell Junctions

David McDonald 1, Li Wu 2, Stacy M. Bohks 3, Vineet N. KewalRamani 2, Derya Unutmaz 3, Thomas J. Hope 1*

1 Department of Microbiology and Immunology, University of Illinois, Chicago, IL 60612, USA.
2 HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
3 Department of Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA.

* To whom correspondence should be addressed. E-mail: thope@uic.edu.

Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface, while the MDDCs concentrated HIV to the same region. We propose that DC-T cell contact facilitates transmission of HIV by locally concentrating virus, receptor and coreceptor during the formation of an infectious synapse.

Science 10.1126/science.1084238
Copyright © 2003 by The American Association for the Advancement of Science. All rights reserved.