Submitted on March 7, 2003
Accepted on April 17, 2003
Recruitment of HIV and Its Receptors to Dendritic Cell-T Cell Junctions
David McDonald 1,
Li Wu 2,
Stacy M. Bohks 3,
Vineet N. KewalRamani 2,
Derya Unutmaz 3,
Thomas J. Hope 1*
1 Department of Microbiology and Immunology, University of Illinois, Chicago, IL 60612, USA.
2 HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
3 Department of Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA.
* To whom correspondence should be addressed. E-mail: firstname.lastname@example.org.
Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface, while the MDDCs concentrated HIV to the same region. We propose that DC-T cell contact facilitates transmission of HIV by locally concentrating virus, receptor and coreceptor during the formation of an infectious synapse.